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Analysis of Edwardsiella piscicida induced transcriptome profiles in macrophages of black rockfish (Sebastes schlegelii) pr ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Ethics statements 2 2.2 Experimental fish and bacteria strain 2 2.3 Cell preparation 2 2.4 Bacteria invasion 2 2.5 Annexin V-FITC/PI stain, lactate dehydrogenase release, and DNA integrity assay 2 2.6 Western blotting 2 2.7 RNA extraction, cDNA synthesis, and quantitative expression analysis 2 2.8 RNA sequencing and DEGs identification 3 2.9 Statistical analysis 3 3 Results 3 3.1 E. piscicida invasion induces HKMs swelling 3 3.2 E. piscicida invasion impaired the membrane integrity of HKMs 3 3.3 E. piscicida invasion caused DNA damage and cleavage of SscGSDMEb in HKMs 3 3.4 Transcriptome sequencing and assemble 3 3.5 Differential expression analysis 3 3.6 GO and KEGG enrichment analysis of DEGs 6 3.7 The immune-related protein interaction networks 7 3.8 Validation of DEGs by qRT-PCR 7 3.9 ROS is essential for pyroptosis and inflammatory response caused by E. piscicida 7 4 Discussion 7 CRediT authorship contribution statement 9 Declaration of Competing Interest 9 Data availability 9 Acknowledgment 9 Reference

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Language

EN

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nonfiction