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Toosendanin and isotoosendanin suppress triple-negative breast cancer growth via inducing necrosis, apoptosis and autophagy 1 1 Introduction 1 2 Materials and methods 2 2.1 Cell line 2 2.2 Natural chemical compounds 2 2.3 Cell viability assay 2 2.4 Propidium iodide (PI) staining assay 2 2.5 Annexin V-FITC and PI double-staining assay 2 2.6 Western-blot analysis 3 2.7 Transmission electron microscope observation 3 2.8 Animals and treatments 3 2.9 Statistical analysis 3 3 Results 3 3.1 TSN and ITSN inhibited the growth of various tumor cells in vitro 3 3.2 TSN and ITSN induced TNBC necrotic death in vitro 3 3.3 TSN and ITSN induced TNBC apoptosis in vitro 3 3.4 TSN and ITSN induced TNBC autophagy in vitro 8 3.5 TSN and ITSN reduced the growth of TNBC in vivo 8 4 Discussion 8 Author statement 11 Declaration of competing interest 11 Acknowledgements 11 Appendix A Supplementary data 11 References 11

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nonfiction