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Investigation of antiproliferative mechanisms of Alstonia angustiloba-silver nanoparticles in skin squamous cell carcinoma (A431 cell line) 1 1 Introduction 1 2 Methodology 2 2.1 Materials and reagents 2 2.2 Plant materials 2 2.3 Preparation of aqueous extract 2 2.4 Biosynthesis of A. angustiloba-AgNPs 2 2.5 Characterisation of biosynthesised A. angustiloba-AgNPs 3 2.6 Cell lines and culture 3 2.7 Cytotoxicity study and long-term antiproliferative effect 3 2.8 Light microscopy 3 2.9 DAPI staining assay 3 2.10 AO/PI double staining assay 3 2.11 Annexin V-FITC/PI apoptotic assay 3 2.12 Analysis of cell cycle 4 2.13 Statistical analysis 4 3 Results and discussion 4 3.1 Biogenic synthesis of Alstonia angustiloba-AgNPs 4 3.2 Characterisation of A. angustiloba-AgNPs 4 3.3 Ultra-High resolution field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX) and transmission electron microscopy (TEM) analysis 4 3.4 Dynamic light scattering (DLS) and fourier transform infrared spectroscopy (FT-IR) 5 3.5 Cytotoxicity study and long-term antiproliferative effect 6 3.6 Morphological assessment of A431 cells using DAPI and AO/PI staining 7 3.7 Annexin V-FITC/PI double stainin

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EN

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nonfiction