Can I read Trigonelline Shields Chondrocytes from Oxidative Damage in Osteoarthritis through Activation of the Keap1/Nrf2/ARE Signaling Pathway on EtoBox?

Trigonelline Shields Chondrocytes from Oxidative Damage in Osteoarthritis through Activation of the Keap1/Nrf2/ARE Signaling Pathway by Chunmei Jiang & Xiaohong He & Aiju Lou & Shiwen He & Qixin Xie & Yuechun Wang & Shan Zhong & Weirong Wu & Qingchun Huang is a book available to read on EtoBox.

What is Trigonelline Shields Chondrocytes from Oxidative Damage in Osteoarthritis through Activation of the Keap1/Nrf2/ARE Signaling Pathway about?

Applied Biochemistry and Biotechnology,AbstractOxidative stress-induced chondrocyte damage is a key contributor to the progression of osteoarthritis (OA). While trigonelline (TG) possesses anti-infammatory and antioxidant activities, its functional role and underlying mechanisms in OA remain unclear. In this study, the human chondrocyte cell line CHON-001 was treated with TG alone or in combination with IL-1β or ML385 for 24 h. Chondrocyte injury-related events were assessed using Cell Counting Kit-8 (CCK-8), fow cytometry with Annexin V-FITC/PI kit, Hoechst staining, the probe 2,7-Dichlorofuorescin diacetate (DCFH-DA), SA-β-gal staining, and SOD and MDA assay kits. Our data revealed that TG alleviated IL-1β-induced infammation, apoptosis, extracellular matrix (ECM) degradation, senescence, and oxidative stress in chondrocytes, accompanied by the downregulation of Keap1 and upregulation of Nrf2, HO-1 and NQO1. ML385 treatment reversed the protective efects of TG against IL-1βinduced injury in chondrocytes. In vivo, the anterior cruciate ligament transection (ACLT) was used to induce a rat OA model, and TG was administered by gavage. OA severity and articular cartilage degradatio

Author

Chunmei Jiang & Xiaohong He & Aiju Lou & Shiwen He & Qixin Xie & Yuechun Wang & Shan Zhong & Weirong Wu & Qingchun Huang

Publisher

x

Published

2025

Language

EN