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Chemical composition, anticancer activities and related mechanisms of the essential oil from Alpinia coriandriodora rhizome 1 1 Introduction 1 2 Materials and methods 2 2.1 Reagents 2 2.2 Plant material 2 2.3 Preparation of essential oil 2 2.4 Composition analysis of EO 2 2.5 Cytotoxic activity 2 2.6 Colony formation assay 2 2.7 Cell cycle assay 3 2.8 Cell apoptosis assay 3 2.8.1 Morphology assay 3 2.8.2 Annexin V-FITC/PI assay 3 2.9 Mitochondrial membrane potential staining 3 2.10 Cell migration and invasion assay 3 2.10.1 Wound healing assay 3 2.10.2 Transwell invasion assay 3 2.11 Western blotting assay 5 2.12 Statistical analysis 5 3 Results 5 3.1 Chemical compounds of EO 5 3.2 Cytotoxic activity of EO 5 3.3 Inhibitory effects of EO on colony formation 5 3.4 EO induced G1 phase cell cycle arrest 6 3.5 EO induced apoptosis of A549 cells 6 3.6 EO induced depolarization of mitochondrial membrane potential 8 3.7 EO-induced apoptosis via mitochondria-mediated pathway 8 3.8 EO inhibited A549 cells migration and invasion 8 4 Discussion 9 5 Conclusion 11 CRediT authorship contribution statement 11 Declaration of Competing Interest 11 Acknowledgment 12 References 12

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Language

EN

Category

nonfiction