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Genome-wide identification and expression analysis of the Hsp gene superfamily in Asian long-horned beetle (Anoplophora gla ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Identification and sequence analysis of the AglaHsp gene family 2 2.1.1 Identification of Hsp gene family 2 2.1.2 Sequence analysis and phylogenetic tree construction 2 2.1.3 Structural features analysis of AglaHsps 2 2.2 Expression analysis of AglaHsp genes 2 2.2.1 Insect collection and temperature treatments 2 2.2.2 Expression analysis of AglaHsp genes in larval overwintering transcriptomes 2 2.2.3 RNA isolation and qPCR analysis of AglaHsp genes 3 2.2.4 Statistical analysis 3 3 Results 3 3.1 Genome-wide identification of Hsp genes in A. glabripennis 3 3.2 Phylogenetic analysis of AglaHsps 4 3.3 AglaHsp gene and protein structure 4 3.4 Expression analysis of AglaHsps under different temperature treatments 4 3.4.1 Expression patterns of AglaHsps during larval overwintering 4 3.4.2 Expression patterns of AglaHsps under low temperature stress 4 4 Discussion 7 4.1 Expansion of the Hsp gene family in A. glabripennis 7 4.2 Structure and environmental adaptability of AglaHsps 7 4.3 Specific AglaHsp genes are impo

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UND

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nonfiction