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Comprehensive investigation of cucumber heat shock proteins under abiotic stress conditions: A multi-omics survey 1 1 Introduction 1 2 Materials and methods 3 2.1 Materials 3 2.2 Identification of Hsp genes in cucumber genome 3 2.3 Chromosomal location of Hsp genes and gene structure 3 2.4 Phylogenetic analysis and identification of conserved motifs 3 2.5 Gene ontology (GO) annotation 3 2.6 Determination of orthologous relations with other plants 3 2.7 Estimation of synonymous and non-synonymous substitution rates 3 2.8 In-silico identification of miRNA’s targeting hsp transcripts 3 2.9 Homology modeling of Hsp proteins 3 2.10 Expression profiling of Hsp genes 3 2.11 Plant material, growth conditions, and stress treatments 4 2.12 RNA extraction and quantitative PCR analysis 4 2.13 Extraction, derivatization processes and GC-MS analysis 4 2.14 Statistical analysis 4 3 Results and discussion 4 3.1 Characterization of CsHsp proteins 4 3.2 Phylogenetic analysis 6 3.3 Gene structure 6 3.4 Gene ontology 7 3.5 Prediction of 3D structure 8 3.6 Gene duplication analyzes orthologous relations and synonymous/non-synonymous substitution rates 9 3.7 miRNA targets 11 3.8 Genome-wide expression p

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Language

UND

Category

nonfiction