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Genome-wide identification and expression analyses of late embryogenesis abundant (LEA) gene family in tobacco (Nicotiana t ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Plant materials and stress treatments 2 2.2 Identification and characterization of LEA genes in Nicotiana tabacum 2 2.3 Sequence analysis and phylogenetic tree construction 2 2.4 Analysis of gene structure and protein conserved motifs 2 2.5 Prediction of protein–protein interaction networks and transcription factor regulatory networks 2 2.6 Expression analysis of NtLEA genes 3 3 Results 3 3.1 Identification and characteristics of NtLEA genes in Nicotiana tabacum 3 3.2 Phylogenetic tree, gene structure and conserved motif analyses of NtLEA genes 3 3.3 Protein-protein interaction network and transcription factor regulatory network of NtLEA genes 4 3.4 Expression pattern of NtLEAs under abiotic stress 4 4 Discussion 5 CRediT authorship contribution statement 8 Declaration of Competing Interest 8 Acknowledgements 8 Appendix A Supplementary data 8 References 9

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Language

UND

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nonfiction