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Molecular characterization, expression and response to immune challenges of 3 members of the toll-like receptor superfamily ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Experimental design and samples collection 2 2.2 In silico identification and molecular cloning of Totlr13 and Totlr23 genes 2 2.3 Sequence analysis and data processing of Totlr13 and Totlr23 genes 2 2.4 Phylogenetic analysis 2 2.5 Gene synteny and structure comparison of Totlr13 and Totlr23 genes 3 2.6 RNA isolation and quantitative PCR 3 2.7 Statistical analysis 4 3 Results 5 3.1 Molecular cloning and sequence analysis of tlr13 and tlr23 genes 5 3.2 Phylogeny of TLRs genes families 6 3.3 Gene synteny and structure comparison 6 3.4 Tissue distribution pattern of Totlr13 and Totlr23 genes 6 3.5 Expression of Totlr13s and Totlr23 in response to different immune challenges 7 4 Discussion 7 CRediT authorship contribution statement 10 Declaration of Competing Interest 10 Data availability 10 Acknowledgments 10 Appendix A Supporting information 11 References 11

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Language

UND

Category

nonfiction