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In vitro digestion and fermentation by human fecal microbiota of polysaccharides from Clitocybe squamulose 1 1 Introduction 1 2 Materials and methods 2 2.1 Materials and reagents 2 2.2 Preparation of CSFP 2 2.3 In vitro simulated saliva, gastric and small intestinal digestion 2 2.4 Determination of physicochemical properties of CSFP and its digestive products 3 2.4.1 Chemical composition and molecular weight distribution 3 2.4.2 Ultraviolet-visible absorption spectra (UV) and Fourier transform infrared spectroscopy (FT-IR) analysis 3 2.4.3 Thermogravimetric analysis 3 2.4.4 Rheological characterization 3 2.4.5 Mean particle size and zeta potential 3 2.4.6 Scanning electron microscopy (SEM) 3 2.5 Evaluation of bioactivities of CSFP and its digestive products 3 2.5.1 In vitro antioxidant activities 3 2.5.2 In vitro α-glucosidase and α-amylase inhibitory effect 3 2.5.3 In vitro binding properties 3 2.6 In vitro fermentation assay 3 2.7 Analysis of CSFP and its fermented fractions 4 2.7.1 Determination of pH, OD600, total carbohydrate, and CR 4 2.7.2 Determination of SCFAs 4 2.7.3 Analysis of gut microbiota 4 2.8 Statistical analysis 4 3 Results and discussion 4 3.1 Effects of in vitro

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UND

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nonfiction