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Dynamic variations in physicochemical characteristics of oolong tea polysaccharides during simulated digestion and fecal fe ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Materials and chemicals 2 2.2 Preparation and isolation of polysaccharides from ‘Wuyi rock’ tea 2 2.3 Simulated digestion of WYP 2 2.4 Microbial fermentation of the indigestible WYP (WYP-I) by human feces 2 2.5 Measurement of variations in physicochemical characteristics of polysaccharides from ‘Wuyi rock’ tea at different diges ... 2 2.5.1 Measurement of CR, digestibility, fermentability, and chemical composition 2 2.5.2 Measurement of free sugars released and monosaccharide composition 2 2.5.3 Measurement of molecular weights 3 2.5.4 Spectrometric analysis 3 2.6 Analysis of gut microbial composition and short chain fatty acids (SCFAs) 3 2.7 Statistical analysis 3 3 Results and discussion 3 3.1 Dynamic variations in physicochemical characteristics of WYP at different simulated digestion stages in vitro 3 3.1.1 Dynamic variation in chemical composition 3 3.1.2 Dynamic variation in molecular weight 3 3.1.3 Dynamic variation in CR 3 3.1.4 Dynamic variation in constituent monosaccharide 4 3.1.5 Dynamic variatio

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UND

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nonfiction