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Influence of encapsulation on the survival of probiotics in food matrix under simulated stress conditions 1 1 Introduction 1 2 Materials and Methods 2 2.1 Materials 2 2.2 Activation of microbial strain 2 2.3 Micro-encapsulation 2 2.4 Characterization of microbeads 2 2.4.1 Size 2 2.4.2 Encapsulation efficiency 2 2.5 Yoghurt fermentation 2 2.6 Physicochemical analysis of yoghurt 2 2.6.1 pH 2 2.6.2 Lactose 2 2.6.3 Acidity 2 2.6.4 Viscosity 3 2.6.5 Syneresis 3 2.7 Yoghurt sensory evaluation 3 2.8 Microbiological analysis 3 2.8.1 B. Bifidium viability in yoghurt 3 2.8.2 Free and encapsulated B. Bifidium viability in simulated gastric conditions (in-vitro) 3 2.8.3 Viability of B. Bifidium in simulated intestinal fluid (in-vitro) 3 2.8.4 Statistical analysis 3 3 Results 3 3.1 Characterization of microcapsules 3 3.1.1 Morphological analysis 3 3.1.2 Encapsulation efficiency 3 3.2 Physicochemical analysis of yoghurt 3 3.2.1 pH and acidity 3 3.2.2 Lactose 3 3.2.3 Viscosity and syneresis 3 3.3 Sensory evaluation 5 3.4 Microbiological analysis 5 3.4.1 B. Bifidium viability in yoghurt 5 3.4.2 Free and encapsulated B. Bifidium viability in simulated gastric conditions and simulated intestinal con

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EN

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nonfiction