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Cordycepin alleviated metabolic inflammation in Western diet-fed mice by targeting intestinal barrier integrity and intesti ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Materials and reagents 2 2.2 Animal experiments. 2 2.3 Biochemical analysis 4 2.4 OGTT 4 2.5 Serum cytokine measurements 4 2.6 Serum and fecal lipopolysaccharide detection 4 2.7 Intestinal permeability analysis 4 2.8 Histopathological analysis 4 2.9 Quantitative polymerase chain reaction analysis 5 2.10 Western blot analysis 5 2.11 Immunofluorescence analysis 5 2.12 Mass spectrometry imaging 5 2.13 Multiplexed gene expression by NanoString nCounter array 5 2.14 DNA extraction and metagenomic analysis 5 2.15 Fecal microbiota transplantation (FMT) 6 2.16 Quantitative analysis of cordycepin in feces by LC-MS/MS 6 2.17 Statistical analysis 6 3 Results 6 3.1 Cordycepin attenuated WD-induced systemic inflammation and metabolic disorders in mice 6 3.2 Cordycepin reduced WD-induced increase in intestinal permeability, intestinal inflammation, and intestinal epithelial c ... 6 3.3 Cordycepin protected the intestinal barrier function by inhibiting the expression of Nox1 and activating the antioxidan ... 11 3.4 Cordyce

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nonfiction