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Hedysari Radix Praeparata Cum Melle repairs impaired intestinal barrier function and alleviates colitis-associated colorect ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Preparation and quality analysis of HR 2 2.2 Animals and experimental protocols 2 2.3 Pathological analysis 2 2.4 Enzyme-linked immunosorbent assay 2 2.5 Immunohistochemistry 2 2.6 Immunofluorescence 3 2.7 16S rRNA gene sequencing 3 2.8 Untargeted metabolomics 3 2.9 Statistical analysis 3 3 Results 3 3.1 Qualitative and quantitative analysis of HR extract 3 3.2 HR suppressed colitis-associated colon tumorigenesis in CAC mice 3 3.3 HR attenuated histopathological injury, the expression of tumor marker and inflammation in CAC mice 4 3.4 HR improved the function of intestinal barrier in CAC mice 5 3.5 HR recovered the compromised gut microbiota in CAC mice 6 3.6 Screening of differential microbiota after treatment of HR in CAC mice 7 3.7 HR treatment regulates fecal metabolic profiling in CAC mice 7 3.8 Pathway enrichment, determination of the key differential metabolites, and identification of fecal biomarkers 8 3.9 Relationship between the intestinal barrier function, microbiota and differential metabolisms v
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- Language
- EN
- Category
- nonfiction
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