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Butyric-lauric acid structural lipid relieves liver inflammation and small intestinal microbial disturbance: In obese male ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Materials 2 2.2 Methods 2 2.2.1 Synthesis of structural lipids (SBL) 2 2.2.2 Simulated digestion of SBL in vitro 2 2.2.3 Animal experiment 2 2.2.3.1 Animals 2 2.2.3.2 Experimental design and sampling 2 2.2.4 Morphological observation of liver and jejunum 2 2.2.5 Detection of serum biochemical indicators 3 2.2.6 Detection of liver biochemical indicators 3 2.2.7 Analysis of fatty acid distribution in liver 3 2.2.8 Quantitative RT-PCR analysis 3 2.2.9 16S rRNA sequencing and analysis 3 2.2.10 Statistical analysis 3 3 Results 3 3.1 SBL improved blood lipid index and reduced serum inflammatory factor levels 3 3.2 SBL alleviated liver lipid metabolism disorders 3 3.3 SBL inhibited liver inflammation 5 3.4 Hepatic lipid metabolism 6 3.5 Small intestinal microbes 7 3.5.1 SBL improved small intestinal microbial diversity 7 3.5.2 Composition and distribution of small intestinal microbes 8 3.5.3 Correlation analysis of small intestinal microbes with liver indicators 10 4 Discussion 10 5 Conclusions 11 Author statement

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nonfiction