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Monascus vinegar protects against liver inflammation in high-fat-diet rat by alleviating intestinal microbiota dysbiosis an ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Materials and reagents 2 2.2 Design of animal experiments 2 2.3 Sample harvesting 2 2.4 Biochemical assays 2 2.5 Histological analysis 2 2.6 SCFAs analysis 2 2.7 Quantitative real-time polymerase chain reaction (qRT-PCR) 3 2.8 Western blot analysis 3 2.9 RNA sequencing 3 2.10 Fecal microbial composition 3 2.11 Statistical analysis 4 3 Results 4 3.1 Effects of MV treatment on signs and serum biochemical parameters of rats 4 3.2 Effects of MV on liver inflammation and histopathology 4 3.3 Effect of MV on the intestinal microbiota composition in HFD-fed rats 4 3.4 Effect of MV pretreatment on the bacterial inflammatory functionality in HFD-fed rats 5 3.5 Effect of MV on intestinal barrier function in HFD-fed rats 5 3.6 Effect of MV on fecal SCFAs levels in HFD-fed rats 6 3.7 Molecular mechanism of MV on HFD-induced hepatitis 6 3.8 Correlations among MV-treated intestinal microbial genus and host inflammation-related parameters 6 4 Discussion 10 5 Conclusion 11 CRediT authorship contribution statement 11 Declara

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nonfiction