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Enriching intracellular macrolides in Escherichia coli improved the sensitivity of bioluminescent sensing systems 1 1 Introduction 1 2 Materials and methods 2 2.1 Development of strains and plasmids 2 2.2 Measurement of intracellular concentration of macrolides 2 2.3 Culture condition and analytical method 2 3 Results and discussion 3 3.1 Selection of host strain and growth condition 3 3.2 Intracellular macrolide enrichment increased sensitivity 3 3.3 Analysis of azithromycin in human serum 4 3.4 Construction of FK506 bioluminescent sensing system and measurement of FK506 in human serum 5 4 Conclusions 7 Author contributions 7 Funding 7 Declaration of competing interest 7 Appendix A Supplementary data 7 References 7
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