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Potassium ion leakage impairs thermotolerance in Corynebacterium glutamicum 1 Materials and methods 2 Bacterial strains and growth conditions 2 Experimental adaptation of a laboratory thermo-sensitive mutant RM-1 2 Measurement of intracellular potassium concentration 2 Measurement of protein concentration 2 Genome sequencing and mapping analysis of laboratory thermo-sensitive RM-1 strain and thermo-adapted C2D strain 2 Sequence data deposition 2 Results 2 Characterization of the thermo-sensitive natural mutant, C. glutamicum RM-1 2 Effect of potassium ion on thermotolerance of C. glutamicum RM-1 2 Effect of ionic strength or osmotic pressure on thermotolerance of C. glutamicum 3 Correlation between intracellular potassium concentration and cell growth 4 Discussion 4 Gene mutations involved in thermo-sensitivity or thermo-tolerance in the RM-1 and C2D strains 4 Correlation between thermotolerance and potassium leakage in C. glutamicum 5 Appendix ASupplementary dataThe following is the Supplementary data to this article:Multimedia component 1Multimedia compon ... 6 References 6

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