Can I read Enhanced Bioreduction of Radionuclides by Driving Microbial Extracellular Electron Pumping with an Engineered CRISPR Platform on EtoBox?

Enhanced Bioreduction of Radionuclides by Driving Microbial Extracellular Electron Pumping with an Engineered CRISPR Platform is a nonfiction available to read on EtoBox.

What is Enhanced Bioreduction of Radionuclides by Driving Microbial Extracellular Electron Pumping with an Engineered CRISPR Platform about?

S2 ## Supplementary Methods Bacterial Cultivation Conditions. Escherichia coli NEB10β and WM3064 were used for plasmid construction and propagation. Shewanella oneidensis MR-1 (NC\_004347.2). was utilized for genome editing and phenotypic validation. E. coli strains were cultivated in Luria-Bertani (LB) medium (10 g/L tryptone, 5 g/L yeast extract, and 10 g/L NaCl) at 37 °C. S. oneidensis strains were cultivated in LB medium or minimal salt medium 1 at 30 °C. When necessary, antibiotics at appropriate concentrations (μg/mL) were added: for E. coli, 50 of kanamycin, 34 of chloramphenicol; for S. oneidensis, 50 of kanamycin, 20 of chloramphenicol. Inducers of IPTG and L-arabinose were added at the concentrations as indicated. For blue-white selection, X-gal was supplemented at a concentration of 100 μg/mL. The cellular growth was assessed by measuring OD600 of cultures with the spectrophotometry method. Genetic Manipulation. PCR amplification was performed using the KOD-Plus-Neo DNA polymerase (Toyobo Co., Japan) for DNA assembly. PCR verification was conducted with 2x Hot-Start Master Mix (Transgene Co., China). Enzymes preparing for Gibson reaction and restriction digestion were pu

Who reads Enhanced Bioreduction of Radionuclides by Driving Microbial Extracellular Electron Pumping with an Engineered CRISPR Platform?

Publisher

American Chemical Society (ACS)

Language

EN

Category

nonfiction