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Differences in recipient ability of uropathogenic Escherichia coli strains in relation with their pathogenic potential 1 1 Introduction 1 2 Materials and methods 2 2.1 Bacterial strains, plasmids and growth conditions 2 2.2 Phylogenetic group, virulence-associated and pOX38 plasmid genes 2 2.3 Antimicrobial susceptibility testing 2 2.4 Bacteriocin production 2 2.5 Bacteriocin insensitivity testing 2 2.6 Conjugation assays 2 2.7 Biofilm biomass assays 2 2.8 Quantification and statistics 3 3 Results 3 3.1 Conjugation frequencies in UPEC strains in plankton and biofilm 3 3.2 Conjugation frequencies in relation to biofilm thickness of recipient UPEC strain 3 3.3 Conjugation frequencies in plankton and biofilm in relation to virulence-associated genes 3 3.4 Conjugation frequency in plankton and biofilm in relation to antibiotic resistance 3 3.5 Conjugation frequency in plankton and biofilm in relation to bacteriocin production and insensitivity 4 3.6 Conjugation frequencies in plankton and biofilm in relation to F- plasmid-associated genes 6 4 Discussion 6 Data statement 7 Declaration of Competing Interest 7 Acknowledgement 7 Appendix A Supplementary data 7 References 7

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Language

UND

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nonfiction