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Hydroxypropyl-beta-cyclodextrin (HP-BCD) inhibits SARS-CoV-2 replication and virus-induced inflammatory cytokines 1 1 Introduction 1 2 Materials and methods 2 2.1 Cell lines and viruses 2 2.2 Ethical statement and isolation of human primary monocytes 2 2.3 Cellular infection with SARS-COV-2 2 2.4 Treatment of cells and viruses with HP-BCD 3 2.5 Cell viability 3 2.6 Cholesterol measurement 3 2.7 Analysis of virus replication by RT-qPCR 3 2.8 Plaque assay 3 2.9 Analysis of ACE2, CD147 and spike proteins expression by flow cytometry and western blotting 3 2.10 Evaluation of cholesterol and dsRNA content by fluorescence microscopy 4 2.11 Analysis of cytokine expression by RT-qPCR 4 2.12 Statistical analysis 4 3 Results 4 3.1 HP-BCD presents a broad antiviral activity against SARS-CoV-2 variants in vitro 4 3.2 HP-BCD antiviral activity does not involve ACE2 depletion 4 3.3 Host cell treatment with HP-BCD reduces the accumulation of SARS-CoV-2 RNA and S protein and restrains virus-induced CPE 7 3.4 HP-BCD restricts SARS-CoV-2 replication and inhibit cytokine production in human alveolar epithelial cell line and prim ... 7 4 Discussion 9 Declaration of interests 12 Acknowledgments 12 Appe

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nonfiction