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Effective inhibition of HCoV-OC43 and SARS-CoV-2 by phytochemicals in vitro and in vivo 1 1 Introduction 1 2 Materials and methods 2 2.1 Cell lines 2 2.2 Viruses 2 2.3 Phytochemicals 2 2.4 Primary screening 2 2.5 Validation by determining infectious HCoV-OC43 titres 2 2.6 Lactate dehydrogenase cytotoxicity assay following SARS-CoV-2 infection 2 2.7 Live/dead microscopy 2 2.8 Determining infectious SARS-CoV-2 titres upon treatment 3 2.9 Mouse studies 3 2.10 Statistical analysis 4 3 Results 4 3.1 Selection of cell lines for analysis of HCoV-OC43 infection 4 3.2 Screening of selected phytochemicals for inhibition of HCoV-OC43-induced cytopathic effect in MRC-5 and HCT-8 cells 5 3.3 Effect of selected phytochemicals on SARS-CoV-2-induced cell death 6 3.4 Lycorine, rottlerin and chebulinic acid, but not capsaicin, effectively reduce SARS-CoV-2 titres 6 3.5 Rottlerin significantly inhibits SARS-CoV-2-induced disease in B6.Cg-Tg(K18-ACE2)2Prlmn/J mice 7 4 Discussion 7 Declarations 8 Supplementary materials 8 References 8

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Language

UND

Category

nonfiction