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Down-expression of the NLRP3 inflammasome delays the progression of diabetic retinopathy 1 1 Introduction 1 2 Materials and methods 2 2.1 Animals 2 2.2 Induction of diabetes 2 2.3 Animal grouping 2 2.4 Immunofluorescence staining 2 2.5 Hematoxylin and eosin(H&E) staining 2 2.6 Immunohistochemistry 2 2.7 FITC-Dextran perfusion 2 2.8 Statistical analysis 2 3 Results 2 3.1 Changes in blood glucose, body weight, urine volume, and water intake in each group 2 3.2 Mcc950 delays the progression of DR in hyperglycemic mice 3 3.3 Down-regulating NLRP3 expression alleviates histopathological alterations in the retinas of mice with DR 4 3.4 Mcc950 reduces the production of IL- 1β in DR 4 3.5 Mcc950 reduces retinal vascular leakage in diabetic mice 4 4 Discussion 4 Funding 6 Studies involving animal subjects 6 Studies involving human subjects 6 Inclusion of identifiable human data 6 CRediT authorship contribution statement 6 Declaration of competing interest 6 Acknowledgements 6 References 6

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EN

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nonfiction