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Anti-CXCL10 monoclonal antibody therapy protects against the diabetic retinopathy in the mouse model induced by streptozotocin 1 1 Introduction 1 2 Materials and methods 2 2.1 Ethical statement 2 2.2 Construction of DR mice models 2 2.3 Drug delivery 2 2.4 Hematoxylin & eosin (HE) staining 2 2.5 Retinal vascular preparations 2 2.6 Measurements of serum VEGF and FGF-2 by ELISA (enzyme-linked immunosorbent assay) 2 2.7 Measurements of oxidative stress indicators 2 2.8 Real-time qPCR 2 2.9 Western blotting 3 2.10 Statistical analysis 3 3 Results 3 3.1 Effect of anti-CXCL10 mAb on the retina pathology of DR mice 3 3.2 Effect of anti-CXCL10 mAb on the levels of inflammatory cytokines in retina of DR mice 3 3.3 Effect of anti-CXCL10 mAb on the oxidative stress in retina of DR mice 3 3.4 Effect of anti-CXCL10 mAb on the number of retinal acellular capillary in DR mice 3 3.5 Effect of anti-CXCL10 mAb on the expression of VEGF and FGF-2 in the retina of DR mice 4 4 Discussion 4 Author contributions 7 Funding 7 Declaration of Competing Interest 7 Acknowledgements 7 References 7

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nonfiction