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Cyanidin-3-galactoside ameliorates silica-induced pulmonary fibrosis by inhibiting fibroblast differentiation via Nrf2/p38/ ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Preparation of C3G 2 2.1.1 Extraction and purification of anthocyanins from A. melanocarpa 2 2.1.2 Purification and identification of C3G 2 2.2 Animal treatment 2 2.3 Histological analysis 2 2.3.1 Hematoxylin and eosin staining 2 2.3.2 Masson’s trichome staining 3 2.3.3 Immunohistochemistry (IHC) staining 3 2.4 HYP assay 3 2.5 Quantitative real-time polymerase chain reaction (RT-qPCR) 3 2.6 ELISA analysis 3 2.7 Cell culture 3 2.8 Cell treatment 3 2.9 Immunofluorescence (IF) staining 3 2.10 Western blot analysis 3 2.11 Assessment of oxidative stress-related indicators 4 2.11.1 MDA assay 4 2.11.2 SOD assay 4 2.11.3 GSH-Px assay 4 2.12 Statistical analysis 4 3 Results 5 3.1 Identification and quantification of C3G 5 3.2 Effects of C3G on SP-induced lung damage 5 3.3 Effect of C3G on the differentiation of lung fibroblasts 5 3.4 Effects of C3G on oxidative stress in lung tissue 5 3.5 Effect of C3G on Nrf2/p38/Akt/NOX4 signal in lung tissue 5 3.6 Effect of C3G on NIH3T3 fibroblasts 5 3.7 Effects of C3G on oxidati

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nonfiction