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LncRNA SNHG6 accelerates hyperoxia-induced lung cell injury via regulating miR-335 to activate KLF5/NF-κB pathway 1 1 Introduction 1 2 Materials and methods 2 2.1 Sample collection 2 2.2 Cell culture 2 2.3 Cell transfection 2 2.4 Hyperoxia-induced BPD model 2 2.5 CCK-8 assay 2 2.6 FACs analysis 2 2.7 The levels of ROS, MDA and SOD detection 2 2.8 ELISa 2 2.9 RT-qPCR 2 2.10 Western blot 3 2.11 Bioinformatics analysis and double luciferase reporter assay 3 2.12 Statistical analysis 5 3 Results 5 3.1 SNHG6 is highly expressed and miR-335 is low expressed in BPD model 5 3.2 SNHG6 knockdown alleviates hyperoxia-induced cell injury 5 3.3 SNHG6 serves as a sponge of miR-335 5 3.4 MiR-335 alleviates hyperoxia-induced cell injury 6 3.5 MiR-335 negatively regulates KLF5 7 3.6 KLF5 knockdown alleviates hyperoxia-induced cell injury via inhibiting NF-κB pathway 7 3.7 SNHG6 mediates hyperoxia-induced cell injury via regulating KLF5/NF-κB pathway 7 4 Discussion 8 CRediT authorship contribution statement 10 Declaration of Competing Interest 10 Acknowledgements 10 Appendix A Supplementary data 10 References 10

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Language

EN

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nonfiction