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The licorice flavonoid isoliquiritigenin attenuates Mycobacterium tuberculosis-induced inflammation through Notch1/NF-κB an ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Reagents and drugs 2 2.2 Antibodies 2 2.3 Cell preparation and culture 4 2.4 CCK-8 assay for cell viability 4 2.5 Bacterial strains and culture 4 2.6 Mtb infection 4 2.7 ELISA 4 2.8 Total RNA isolation and Real-Time PCR 4 2.9 DAPT treatment 4 2.10 Western blot 4 2.11 Extraction of supernatant proteins 4 2.12 Confocal microscopy 4 2.13 Statistical analysis 5 3 Results 5 3.1 The effects of ISL on the viability of Raw264.7 cells and murine primary peritoneal macrophages 5 3.2 ISL decreased the production of proinflammatory cytokines induced by Mtb infection in murine primary peritoneal macroph ... 6 3.3 ISL inhibited Mtb-induced activation of NLRP3 inflammasome in murine primary peritoneal macrophages and Raw264.7 cells 6 3.4 ISL inhibited Mtb-induced activation of GSDMD in murine primary peritoneal macrophages and Raw264.7 cells 7 3.5 ISL inhibited Mtb-induced NF-κB activation 7 3.6 ISL inhibited the activation of the PI3K/Akt signaling pathway 8 3.7 ISL suppressed the phosphorylation of Akt and p65 by down

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nonfiction