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Integrated metabolomic and transcriptomic analysis identifies benzo[a]pyrene-induced characteristic metabolic reprogrammin... 1 1. Introduction 2 2. Materials and methods 2 2.1. Materials 2 2.2. Cell culture and treatment 2 2.3. Determination of neutral lipids 2 2.4. Sample collection and preparation for the metabolomics approach 2 2.5. Instrumental analysis for the metabolomics approach 3 2.6. Data preprocessing for the metabolomics approach 3 2.7. RNA isolation, cDNA library construction, and sequencing 3 2.8. Data processing for the transcriptomic approach 3 2.9. Detection of ROS 3 2.10. Cell treatment and subsequent RT-PCR analysis 3 2.11. Statistical analysis 3 3. Results 3 3.1. BaP exposure induces lipid accumulation and disturbs the metabolic profiling of macrophages 3 3.2. BaP exposure induces metabolic reprogramming in macrophages 4 3.3. BaP exposure induces amino acid metabolic reprogramming in macrophages 4 3.4. BaP exposure induces lipid metabolic reprogramming in macrophages 4 3.5. BaP exposure induces nucleotide and carbohydrate metabolic reprogramming in macrophages 5 3.6. Significant correlations of the metabolic reprogramming with lipid accumulation in BaP-exposed

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nonfiction