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Liraglutide, a glucagon-like peptide-1 receptor agonist, induces ADAM10-dependent ectodomain shedding of RAGE via AMPK acti ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Reagents 2 2.2 Cell culture and treatments 2 2.3 Cell viability assay 2 2.4 Transfection with siRNA 2 2.5 Western blot analysis 2 2.6 Measurement of cytosolic free Ca2+ using Fluo-4 AM 2 2.7 Immunofluorescence staining 3 2.8 Statistical analysis 4 3 Results 4 3.1 Liraglutide and AGE-BSA effects on the viability of HAECs 4 3.2 AGE-BSA stimulates ICAM-1 expression via RAGE, whereas liraglutide suppresses this effect of AGE-BSA 4 3.3 Liraglutide induces ectodomain shedding of RAGE 4 3.4 Liraglutide stimulates extracellular Ca2+ influx via L-type calcium channels 4 3.5 Liraglutide-induced RAGE ectodomain shedding is dependent on extracellular Ca2+ influx via L-type calcium channels 5 3.6 ADAM10 is implicated in the ectodomain shedding of RAGE 5 3.7 AMPK activation is required for the translocation of ADAM10 to the cell surface following liraglutide treatment 5 4 Discussion 6 5 Conclusion 10 CRediT authorship contribution statement 10 Declaration of competing interest 10 Acknowledgements 10 References 10

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nonfiction