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G PROTEIN-COUPLED RECEPTOR SCREENING ASSAYS : methods and protocols. by Sofia Aires M. Martins,Duarte Miguel F. Prazeres (eds.) is a nonfiction available to read on EtoBox.

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Preface Key Outcomes of This Edition Editors´ Final Remarks Acknowledgments Contents Contributors Chapter 1: Isolation of Lipid Rafts by the Detergent-Based and Non-detergent-Based Methods for Localization of GPCRs with Immu... 1 Introduction 1.1 GPCR Signaling and Trafficking 1.2 Perturbation of Raft Stability 1.3 Changing the Cholesterol Content 1.4 Fluorescence Imaging 2 Materials 2.1 Detergent-Based Method 2.2 Detergent-Free Method 2.3 Cell Handling 2.4 Localization of GPCRs in Lipid Rafts 3 Methods 3.1 Isolation of Lipid Rafts 3.2 Isolation of Lipid Rafts Using the Detergent-Based Method 3.3 Isolation of Lipid Rafts Using the Detergent-Free Method 3.4 Localization of GPCR in Lipid Rafts Using Laser Scanning Confocal Microscopy 4 Notes References Chapter 2: Detection of GPCR mRNA Expression in Primary Cells Via qPCR, Microarrays, and RNA-Sequencing 1 Introduction 2 Materials 2.1 Cell Lines 2.2 Equipment 2.3 Reagents and Kits 2.4 Consumables 2.5 Software Tools 3 Methods 3.1 mRNA Isolation from Cultured Cells 3.2 cDNA Synthesis 3.3 qPCR Primer Design (Bioinformatics Protocol) 3.4 Primer Validation 3.5 Independent qPCR 3.6 qPCR-Based TaqMan Array 3.7 RNA-Sequencing Data Analysis f

Who reads G PROTEIN-COUPLED RECEPTOR SCREENING ASSAYS : methods and protocols.?

Author

Sofia Aires M. Martins,Duarte Miguel F. Prazeres (eds.)

Publisher

Springer US : Imprint: Humana

Published

2021

Language

EN

ISBN

9781071612200

Category

nonfiction

Subjects

Science, Biology, Molecular