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Human periodontal ligament stem cell encapsulation in alginate-fibrin-platelet lysate microbeads for dental and craniofacia ... 1 1 Introduction 1 2 Materials and methods 2 2.1 hPDLSC isolation from extracted human teeth 2 2.2 Cell proliferation assay 3 2.3 hPDLSCs encapsulation in alginate microbeads 3 2.4 Viability of hPDLSCs inside alginate microbeads 3 2.5 ALP activity assay 3 2.6 Quantitative real-time PCR 3 2.7 Bone mineral synthesis by hPDLSCs 4 2.8 Statistical analysis 4 3 Results 4 3.1 Growth-promoting effects of hPL on hPDLSCs 4 3.2 Encapsulated hPDLSC proliferation and viability in microbeads 4 3.3 ALP activity of hPDLSCs 5 3.4 Osteogenic gene expression 5 3.5 ALP and Alizarin red staining of minerals synthesized by hPDLSCs 5 4 Discussion 5 5 Conclusion 9 CRediT authorship contribution statement 9 Declaration of Competing Interest 11 Acknowledgments 11 References 11
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Common subject areas: history, science, philosophy, social sciences.
- Language
- EN
- Category
- nonfiction
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