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Effects of different courses of moxibustion treatment on intestinal flora and inflammation of a rat model of knee osteoarthritis 1 1 Introduction 1 2 Materials and methods 2 2.1 Animals 2 2.2 Study design 2 2.3 Study procedure 2 2.4 Intervention methods 2 2.5 Sampling 2 2.6 Histopathologic examination 3 2.6.1 Hematoxylin and eosin staining 3 2.6.2 Detection of inflammatory factors interleukin-1β, tumor necrosis factor-α and interleukin-10 in the serum 3 2.7 The 16S rRNA-assisted high-throughput sequencing analysis 3 2.8 Statistical analysis 3 3 Results 3 3.1 General histologic observations 3 3.2 Cartilage tissue pathologic analysis 3 3.2.1 HE staining of cartilage tissue 3 3.2.2 Mankin score of cartilage tissue 3 3.3 Levels of IL-1β, TNF-α and IL-10 in the serum of rats 3 3.4 The effect of different courses of moxibustion on the intestinal flora of KOA model rats 4 3.4.1 Sequencing data quality assessment rarefaction curve 4 3.4.2 Species distribution column 4 3.4.3 α-Diversity analysis 5 3.4.4 β-Diversity analysis 5 3.4.5 Species abundance heatmap 6 3.4.6 Biomarker differences between groups 6 4 Discussion 6 5 Conclusion 8 Authors’ contribution 8 Funding 8 Declaration of competing

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Language

UND

Category

nonfiction