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ZnO nanoparticles attenuate polymer-wear-particle induced inflammatory osteolysis by regulating the MEK-ERK-COX-2 axis 1 1. Introduction 1 2. Materials and methods 2 2.1. Material characterisation 2 2.2. Cell culture 2 2.3. ELISA 2 2.4. Real-time PCR 2 2.5. Western blot analysis 2 2.6. Air pouch mouse model 2 2.7. Haematoxylin & eosin and immunohistochemical staining 3 2.8. Cell mean density 3 2.9. In vivo evaluation of osteolysis 3 2.9.1. Rat osteolysis model 3 2.9.2. Microcomputed tomography (micro-CT) and section staining 4 2.9.3. H&E and TRAP staining 4 2.9.4. Statistical analysis 4 3. Results and discussion 6 3.1. ZnO NPs are not cytotoxic and do not induce a significant cellular inflammatory response at low concentration in vitro 6 3.2. ZnO NPs attenuate polymer-particle-induced inflammation in vitro 6 3.3. ZnO NPs (5 μg/mL) attenuate polymer particle-induced inflammation and inflammatory osteolysis in vivo 7 3.4. ZnO NPs regulate p-MEK/p-ERK and COX-2 expression induced by polymer particles in vitro and in vivo 7 4. Conclusion 9 Declaration of competing interest 9 Acknowledgements 9 Appendix A. Supplementary data 9 References 9

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Language

UND

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nonfiction