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Zinc oxide nanoparticles attenuate prepubertal exposure to cisplatin- induced testicular toxicity and spermatogenesis impai ... 1 1 Introduction 1 2 Material and methods 2 2.1 Chemicals 2 2.2 Zinc oxide nanoparticles preparation and characterization 2 2.3 Animals 2 2.4 Treatment groups 2 2.5 Biochemical study 2 2.6 Evaluation of Sperm Quality 2 2.7 Assessment of Sperm DNA damage 2 2.8 Gene expression 2 2.9 Histological and Immunohistochemical examination 3 2.10 Ultrastructural study 3 2.11 Statistical Analysis 3 3 Results 3 3.1 Characterization of ZnO-NPs 3 3.2 Bodyweight 3 3.3 Effect of ZnO-NPs on Oxidative stress markers 3 3.4 Effect of ZnO-NPs on Sperm analysis 3 3.5 Comet assay 3 3.6 Effect of ZnO-NPs on the expression of BTB, anti-apoptotic, pro-inflammatory, and protamination markers 4 3.7 Effect of ZnO-NPs on testis histopathology 4 3.8 Ultrastructural analysis 4 4 Discussion 6 5 Conclusion 11 Declaration of Competing Interest 11 References 11

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nonfiction