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A glutathione-responsive silica-based nanosystem capped with in-situ polymerized cell-penetrating poly(disulfide)s for precisely modulating immuno-inflammatory responses 1 1 Introduction 2 2 Materials and methods 2 2.1 Chemicals 2 2.2 Synthesis of red-fluorescent MSNs with different modifications 2 2.3 Characterization of the synthesized nanoparticles 3 2.4 The encapsulation of BE and its GSH-responsive releasing from RC 3 2.5 Cell culture 4 2.6 Cytotoxicity and intracellular radical oxygen species (ROS) assays 4 2.7 Confocal microscopy and transmission electron microscopy 4 2.8 Immunofluorescence staining (IFS) 5 2.9 Enzyme-linked immunosorbent assay (ELISA) 5 2.10 Intracellular GSH levels 5 2.11 NF-κB array and western blot analysis 5 2.12 Statistical analysis 5 3 Results and discussions 5 3.1 Characterization of the as-synthesized MSNs 5 3.2 Cell viability and cellular internalization of nanoparticles 8 3.3 RC and RB modulate immuno-inflammatory responses via NF-κB signaling pathway 10 4 Conclusions 12 CRediT authorship contribution statement 13 Declaration of Competing Interest 13 ack23 13 Acknowledgments 13 Funding Sources 13 Appendix A Supplementary material 13 References 13

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Language

UND

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nonfiction