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LncRNA NORAD deficiency alleviates kidney injury in mice and decreases the inflammatory response and apoptosis of lipopolys ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Animals 2 2.2 AKI mouse model 2 2.3 Cell culture and transfection 3 2.4 Hematoxylin-eosin (H&E) staining 3 2.5 TUNEL staining 3 2.6 RT-qPCR 4 2.7 Western blotting 4 2.8 Luciferase reporter assay 4 2.9 Enzyme linked immunosorbent assay (ELISA) 4 2.10 Renal function measurement 5 2.11 Statistical analysis 5 3 Results 5 3.1 NORAD is highly expressed in AKI 5 3.2 NORAD deficiency hinders the sepsis-induced AKI progression through reducing inflammation and apoptosis 5 3.3 NORAD inhibits HK-2 cell inflammation and apoptosis after LPS stimulation 5 3.4 MiR-577 is a key downstream gene of NORAD 6 3.5 GOLPH3 is identified as a direct target of miR-577 6 3.6 NORAD regulates the LPS-stimulated inflammation and apoptosis of HK-2 cells by GOLPH3 7 4 Discussion 7 Declaration of Competing Interest 8 Acknowledgements 8 Funding 8 Data Availability Statement 8 Appendix A Supplementary material 8 References 8
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It is typically read by self-directed learners exploring a subject in depth.
Common subject areas: history, science, philosophy, social sciences.
- Language
- EN
- Category
- nonfiction
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