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Quantification of nimetazepam and its metabolite in human hair samples using liquid chromatography–tandem mass spectrometry ... 1 1 Introduction 1 2 Case history 2 3 Materials and methods 2 3.1 Chemicals and reagents 2 3.2 Instruments and column 2 3.3 Sample pretreatment 3 3.4 Preparation of standard solutions 3 3.5 Identification and quantification of the target compound 3 3.6 Method validation 3 3.6.1 Selectivity 3 3.6.2 Calibration curve regression equations 3 3.6.3 Precision and accuracy of the method 3 3.6.4 Extraction recoveries (process efficiencies) and matrix effects 3 3.6.5 Stability 5 4 Results and discussion 5 4.1 Identification of nimetazepam and 7-aminonimetazepam 5 4.2 Quantification of nimetazepam and 7-aminonimetazepam in hair samples 5 4.3 Validation of quantification 5 4.4 Discussion 6 5 Conclusion 7 Declaration of Competing Interest 7 Acknowledgments 7 References 7

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Language

UND

Category

nonfiction