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Liquid chromatography-tandem mass spectrometry (LC-MS/MS) based assay for the simultaneous quantification of 18-hydroxycort ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Reagents and instruments 2 2.2 Sample preparation 2 2.2.1 Preparation of stock and working solutions 2 2.2.2 Preparation of sample 2 2.2.3 SPE 2 2.3 LC-MS/MS procedure 2 2.4 Method validation 2 2.4.1 Calibration linearity 2 2.4.2 LLOQ 2 2.4.3 Specificity and matrix effect 3 2.4.4 Intra- and inter-day precisions 3 2.4.5 Recovery 3 2.4.6 Stability 3 2.5 Reference values 3 3 Results 3 3.1 Method development 3 3.2 Method validation 3 3.3 Reference values 4 4 Discussion 4 5 Conclusions 5 Author contributions 5 Funding 5 Employment or leadership 5 Honorarium 5 Declaration of Competing Interest 5 Acknowledgement 5 Appendix A Supplementary material 5 References 5
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- Language
- EN
- Category
- nonfiction
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