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The metabolome of pink-footed goose: Heavy metals and lipid metabolism 1 1 Introduction 1 2 Method 2 2.1 Field sampling 2 2.2 Element analysis 2 2.3 Metabolomics 2 2.3.1 Chemicals, reagents and materials 2 2.3.2 Metabolite extraction 2 2.3.3 Liquid chromatography-high resolution tandem mass spectrometry 3 2.4 Metabolite identification 3 2.5 Data analysis 3 2.5.1 Data analysis in Thermo ScientificTM Compound DiscovererTM 3 2.5.2 Statistical analysis 3 3 Results 3 3.1 Heavy metal concentrations in pink-footed geese 3 3.2 Metabolome profiles of pink-footed geese 4 3.3 Correlation between heavy metals and significant metabolites 4 4 Discussion 5 4.1 Heavy metal toxic thresholds 5 4.2 Heavy metal exposure and the metabolome 5 4.2.1 Cadmium 6 4.2.2 Chromium 6 4.2.3 Mercury 6 4.2.3 Lead 6 4.3 Metabolic pathways affected by heavy metals 6 4.4 Considerations for migratory birds 7 5 Conclusion 7 Credit author statement 7 Declaration of competing interest 7 Data availability 7 Acknowledgments 7 Appendix A Supplementary data 7 References 7

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Language

UND

Category

nonfiction