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Green tea polyphenols inhibit growth, pathogenicity and metabolomics profiles of Streptococcus suis 1 1 Introduction 1 2 Materials and methods 2 2.1 Quantification of GTP extract 2 2.2 Bacterial strains and growth conditions 2 2.3 Growth curves of SC19 against GTP at different concentration 2 2.4 TEM and SEM analysis of bacterial cellular morphology 2 2.5 Hemolytic assay 2 2.6 Infection of Galleria mellonella larvae 2 2.7 Determination of growth and virulence related genes expression by qRT-PCR 2 2.8 Metabolomics analysis 2 2.8.1 Sample collection, preparation and metabolite extraction 2 2.8.2 UPLC-Q-TOF/MS analysis 3 2.8.3 Data processing 3 2.9 Statistical analysis 3 3 Results 3 3.1 EGCG, EGC, ECG, GCG and EC quantitative determination 3 3.2 GTP inhibit the growth of SC19 in vitro 3 3.3 The effect of GTP on bacterial internal and external structure 3 3.4 GTP decrease the hemolytic activity and virulence of SC19 3 3.5 GTP inhibit the expression of growth and virulence related genes of SC19 3 3.6 Metabolic profiling and KEGG enrichment analysis of GTP treated SC19 4 4 Discussion 4 5 Conclusions 6 Author contributions 6 Funding 6 Declaration of competing interest 7 Acknowledgements 7

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UND

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nonfiction