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Impact of simulated in vitro gastrointestinal digestion on bioactive compounds, bioactivity and cytotoxicity of melon (Cucu ... 1 1 Introduction 1 2 Material and methods 2 2.1 Chemicals and reagents 2 2.2 Preparation of melon peel juice (MPJ) 2 2.3 In vitro simulated gastrointestinal digestion 2 2.3.1 Mouth simulated digestion 2 2.3.2 Stomach simulated digestion 2 2.3.3 Gut simulated digestion 2 2.3.4 Small intestine absorption – dialysis 2 2.4 Accessibility and stability of melon bioactive compounds through in vitro gastrointestinal digestion 3 2.4.1 Recovery and accessibility index 3 2.5 Total phenolic content (TPC) 3 2.6 Antioxidant activity determination by ABTS and DPPH 3 2.7 Identification and quantification of polyphenols by HPLC 3 2.8 Identification and quantification of sugars and organic acids by HPLC 3 2.9 Prebiotic potential 3 2.9.1 Growth curves via microplate assay 3 2.10 Evaluation of cytotoxicity: metabolic activity assay 4 2.10.1 Cell line growth conditions 4 2.10.2 Prestoblue assay 4 2.11 Statistical analysis 4 3 Results and discussion 4 3.1 Stability and accessibility of bioactive compounds of melon peel juice during in vitro GIT digestion 4 3.1.1 Soluble sugars
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- Language
- EN
- Category
- nonfiction
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