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Temperature responsive hydrogel for cells encapsulation based on graphene oxide reinforced poly(N- isopropylacrylamide)/hyd ... 1 1 Intruduction 1 2 Materials and methods 3 2.1 Materials 3 2.2 Synthesis of hydroxyethyl chitosan (HECS) 3 2.3 Synthesis of the dithiol-modified graphene oxide (t-GO) 3 2.4 Synthesis of pNiPAM polymer 3 2.5 Preparation of pNiPAM/HECS (pNH) hydrogels 3 2.6 Preparation of pNiPAM/HECS/t-GO (pNHG) hydrogels 3 2.7 Transmission electron microscopy (TEM) 3 2.8 X-ray photoelectron spectroscopy analyzer (XPS) 3 2.9 Fourier-transform infrared spectroscopy (FT-IR) 3 2.10 Scanning electron microscopy (SEM) 3 2.11 Nuclear magnetic resonance (NMR) 3 2.12 Differential scanning calorimeter (DSC) 3 2.13 Spectrophotometric measurement 4 2.14 Rheological measurement 4 2.15 Cell culture 4 2.16 In vitro cell viability 4 2.17 Cells-laden pNHG hydrogels fabrication 4 2.18 In vitro experiments 4 3 Results and discussion 4 4 Conclusion 6 Author contributions 6 CRediT authorship contribution statement 6 Declaration of Competing Interest 6 Acknowledgements 7 Conflicts of interest 7 Appendix A Supporting information 7 References 7
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- Language
- EN
- Category
- nonfiction
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