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Modified nanofiber containing chitosan and graphene oxide-magnetite nanoparticles as effective materials for smart wound dr ... 1 1 Introduction 1 2 Experimental 2 2.1 Materials 2 2.2 Synthesis of chitosan nanoparticle 2 2.3 Preparation of graphene oxide 2 2.4 Synthesis of graphene oxide-magnetite(Fe3O4) nanocomposite 3 2.5 Preparation of electrospinning solution 3 2.6 Drug loading in chitosan nanoparticles 3 2.7 Drug loading in graphene oxide-magnetite nanocomposite 4 2.8 Crosslinked of nanofiber membranes 4 2.9 Characterization 4 2.10 In vitro drug release profile 4 2.11 Degradability test 4 2.12 Swelling degree (SD %) 4 2.13 Antimicrobial tests 4 2.14 MTT assay 4 3 Results and discussion 4 3.1 Investigation of the synthesis of nanoparticles and nanofiber membranes 4 3.1.1 FTIR 4 3.1.2 XRD 5 3.1.3 DSC 6 3.1.4 SEM 6 3.1.5 TEM 7 3.1.6 DLS 7 3.2 Investigation of the nanoparticles and nanofiber membranes in vitro 7 3.2.1 Investigation of drug loading and encapsulation 7 3.2.2 Investigation of in vitro release profile of drug from nanofiber membranes 7 3.2.3 Evaluation of degradability and degree of swelling of nanofiber membranes in vitro 8 3.2.4 Antibacterial activity 10 3.2.5 Cell

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EN

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nonfiction