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Co-exposure of polycarbonate microplastics aggravated the toxic effects of imidacloprid on the liver and gut microbiota in mice 1 1 Introduction 1 2 Materials and methods 2 2.1 Chemicals 2 2.2 Animals and experimental schemes 2 2.3 Histopathology analysis 2 2.4 Biochemical parameter analysis 2 2.5 Extraction and determination of imidacloprid in liver 3 2.6 16S rRNA gene sequencing of intestinal microbiota and data analyses 3 2.7 Statistical analyses 4 3 Results and discussion 4 3.1 Body weight changes of mice after exposure to PC and/or IMI 4 3.2 Toxic effect of PC and/or IMI exposure on liver 5 3.3 Oxidative stress of liver in mice after exposure of PC and/or IMI 5 3.4 Effects of PC and/or IMI on the structure of gut 5 3.5 Effects of PC and/or IMI on the composition of the intestinal microbiota 6 3.6 Changes in the predicted functional capabilities of the mice gut 8 4 Discussion 8 5 Conclusion 10 CRediT authorship contribution statement 10 Declaration of Competing Interest 10 Data availability 10 Acknowledgements 10 Appendix A Supporting information 10 References 10

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Language

UND

Category

nonfiction