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Polystyrene microplastics with different sizes induce the apoptosis and necroptosis in liver through the PTEN/PI3K/AKT/auto ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Animals and PS-MPs exposure 2 2.2 Cells culture and PS-MPs treatment 2 2.3 Transmission electron microscope (TEM) 2 2.4 TdT-mediated dUTP Nick-end labeling (TUNEL) staining 2 2.5 Immunohistochemistry 2 2.6 Immunofluorescence staining 3 2.7 Flow cytometry analysis 3 2.8 Acridine orange/ethidium bromide (AO/EB) dual staining 3 2.9 Dansylcadaverine (MDC) staining 3 2.10 RNA extraction and real time quantitative PCR (RT-qPCR) 3 2.11 Protein extraction and Western blotting 3 2.12 Statistical analysis 3 3 Results 3 3.1 Effect of PS-MPs exposure on liver apoptosis in mice 3 3.2 Effect of PS-MPs exposure on the necroptosis of liver in mice 3 3.3 Effect of PS-MPs treatment on the apoptosis and necroptosis of hepatocytes in vitro 5 3.4 Effect of PS-MPs treatment on the expression of apoptosis and necroptosis-related genes of hepatocytes in vitro 6 3.5 Effect of PS-MPs on the expression of the genes related to PTEN/PI3K/AKT pathway in vivo and in vitro 6 3.6 Effect of PS-MPs on liver autophagy in mice 6 3.7 Effect of P

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UND

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nonfiction