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\advance \chk@titlecnt \@ne Genetically engineered pH-responsive silk sericin nanospheres with efficient therapeutic effect on ulcerative colitis\global \chk@titlecnt =\z@ 1 1 Introduction 1 2 Materials and methods 2 2.1 Materials 2 2.2 Optimize the production of rhLF proteins in cocoons 2 2.3 Histological examination of rhLF in the cocoon silks 3 2.4 Fabrication of sericin nanospheres 3 2.5 Fourier transform infrared spectroscopy (FTIR) assay 4 2.6 X-ray diffraction (XRD) assay 4 2.7 Scanning electron microscope (SEM) and transmission electron microscope (TEM) analysis 4 2.8 Release characteristics of rhLF in the SS-NS-rhLF 4 2.9 Stability analysis of the SS-NS-rhLF 4 2.10 Cell uptake efficiency of rhLF 4 2.11 Anti-inflammatory activity of SS-NS-rhLF in vitro 4 2.12 Treatment of colitis in vivo 4 2.13 Determination of MPO, IL2, IL6 and IL10 in colon tissues 5 2.14 H&E staining and histological evaluation of colitis 5 2.15 Immunofluorescence detection of colonic p65 protein 5 2.16 Effects of SS-NS-rhLF on intestinal microorganisms 5 2.17 Detection of SS-NS-rhLF biodistribution in vivo 5 2.18 In vivo detection of rhLF colonic absorption 5 2.19 The toxicity of SS-NS-rhLF assessment

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nonfiction