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\advance \chk@titlecnt \@ne Platelets and hemostatic proteins are co-localized with chronic neuroinflammation surrounding implanted intracortical microelectrodes\global \chk@titlecnt =\z@ 1 1 Introduction 1 2 Methods 2 2.1 Non-functional multi-shank silicon microelectrode fabrication 2 2.2 Animal use and surgical procedures 2 2.3 Platelet and hemostatic protein immunohistochemistry (IHC) 3 2.4 Immunohistochemistry and tissue processing 3 2.5 Immunohistochemistry analysis 3 2.6 Statistics 4 3 Results 4 3.1 Immunohistochemistry for type IV collagen and CD41+ platelets 4 3.2 Immunohistochemistry for CD62p+ platelets and von Willebrand Factor 4 3.3 Immunohistochemistry for fibrinogen and immune-activated astrocytes 5 3.4 Immunohistochemistry for immune-activated microglia 5 4 Discussion 6 4.1 Local neurovasculature remains in a thromboinflammatory state 6 4.2 Initial vascular damage does not fully resolve and leads to chronic impairments to the blood-brain barrier 9 4.3 Bloodborne platelets may influence neuroinflammation after microelectrode insertion 9 5 Conclusion 10 Declaration of Competing Interest 10 Acknowledgements 10 Supplementary materials 10 References 10

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UND

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nonfiction