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\advance \chk@titlecnt \@ne Fucoidan-loaded nanofibrous scaffolds promote annulus fibrosus repair by ameliorating the inflammatory and oxidative microenvironments in degenerative intervertebral discs\global \chk@titlecnt =\z@ 1 1 Introduction 1 2 Materials and Methods 2 2.1 Materials 2 2.2 Protection of fucoidan on LPS-treated AFCs 2 2.3 Preparation and characterizations of F-PECUU nanofibrous scaffolds 3 2.3.1 Preparation of F-PECUU nanofibrous scaffolds 3 2.3.2 Characterization of F-PECUU nanofibrous scaffolds 3 2.4 In vitro analysis 3 2.4.1 Isolation and culture of rats AFCs 3 2.4.2 Cell viability 3 2.4.3 Cell morphology visualization 3 2.4.4 Cytoskeleton staining 3 2.4.5 Cell proliferation 3 2.4.6 Measurement of ROS production 4 2.4.7 RNA isolation and qPCR analysis 4 2.4.8 Western blotting 4 2.4.9 Immunofluorescence 4 2.5 In vivo analysis 4 2.5.1 Ethical aspects and animals 4 2.5.2 Surgical procedures 4 2.5.3 X-ray and MRI 5 2.5.4 Histological staining 5 2.5.5 Immunohistochemistry 5 2.5.6 Immunofluorescence 5 2.5.7 The mechanical test of IVDs 5 2.6 Statistical analysis 5 3 Results 5 3.1 Fucoidan inhibits LPS-induced inflammatory and oxidative stress in AFCs 5 3.2 Fabrication

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nonfiction