Opening book details…
Can I read Catalogue record on EtoBox?
Catalogue record is a nonfiction available to read on EtoBox.
What is Catalogue record about?
A sensitive and less cytotoxic assay for identification of proliferating T cells based on bioorthogonally-functionalized ur ... 1 1 Introduction 1 2 Methods 2 2.1 PBL and moDC generation 2 2.2 Cryopreservation of PBLs and moDCs 3 2.3 MLR reaction and bead stimulation 3 2.4 CellTrace Far Red loading 3 2.5 EdU incorporation and detection 3 2.6 Antibody staining and flow cytometry 3 2.7 Confocal microscopy 3 2.8 Cytokine secretion detection with ELISA 3 3 Results 5 3.1 EdU-based protocol preserves T cell viability 5 3.2 EdU facilitates sensitive detection of cytokine production 7 3.3 EdU-based protocol is more sensitive for detecting cell proliferation 7 3.4 EdU facilitates robust detection of T cell activation markers 7 4 Discussion 7 Funding 10 Data availability 10 Declaration of Competing Interest 10 Appendix A Supplementary data 10 References 10
Who reads Catalogue record?
It is typically read by self-directed learners exploring a subject in depth.
Common subject areas: history, science, philosophy, social sciences.
- Language
- EN
- Category
- nonfiction
Similar books
- Chemical Composition, Anticancer Activities and Related Mechanisms of the Essent
- Liglaurates A–E, Cytotoxic Bis(lauric Acid-12yl)lignanoates from the Rhizomes Of
- Bioprospecting Dodonaea viscosa Jacq
- Berberine-loaded MSC-derived sEVs Encapsulated in Injectable GelMA Hydrogel For
- Fluorescence Detection of Staphylococcus Aureus Using Vancomycin Functionalized
- Wild Honey: the Untitled Play — Anton Chekhov (2017)