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Liglaurates A–E, cytotoxic bis(lauric acid-12yl)lignanoates from the rhizomes of Drynaria roosii Nakaike 1 1 Introduction 1 2 Results and discussion 1 2.1 Isolation and structure elucidation 1 2.2 Anti-inflammatory activity 3 2.3 Cytotoxic activity 3 2.4 Anti-oxidant activity 4 3 Conclusions 4 4 Experimental 4 4.1 General experimental procedures 4 4.2 Plant material 4 4.3 Extraction and isolation 5 4.4 Synthesis 7 4.4.1 Synthesis of compound (±)-1 7 4.4.2 Synthesis of compound (±)-2 8 4.4.3 Methylation of 1 8 4.4.4 Methylation of 2–5 9 4.4.5 Hydrolysis of 1a 9 4.4.6 Hydrolysis of 2a-5a 9 4.5 Characterization data 9 4.5.1 Liglaurate A (1) 9 4.5.2 Liglaurate B (2) 9 4.5.3 Liglaurate C (3) 9 4.5.4 Liglaurate D (4) 10 4.5.5 Liglaurate E (5) 10 4.6 Theoretical ECD calculation 10 4.7 In vitro cytotoxic assay 10 4.8 In vitro anti-inflammatory assay 10 4.9 Anti-oxidant assay 10 4.9.1 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay 10 4.9.2 Oxygen radical absorbance capacity (ORAC) assay 10 Declaration of competing interest 10 Acknowledgments 10 Appendix A Supplementary data 10 References 10

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